Figure 5
The levels of signaling proteins in mammary tumors from MTB-IGFIR, MTB-IGFIR/Akt1 -/- and MTB-IGFIR/Akt2 -/- mice. Western blot for IGF-IR, Akt1, Akt2, phosphorylated Akt (pAkt; Ser473), phosphorylated Erk (pErk), Erk, phosphorylated Stat3 and Stat3 in (A) mammary tumors from MTB-IGFIR transgenic mice compared to MTB-IGFIR/Akt1−/− mice or (B) mammary tumors from MTB-IGFIR transgenic mice compared to MTB-IGFIR/Akt2−/− mice. Each lane represents tissue from a different animal and the relative abundance of each protein is presented in the bar graph below the western. *p<0.05 as determined by a Student’s T-test.