Figure 1From: Screening for phenotype selective activity in multidrug resistant cells identifies a novel tubulin active agent insensitive to common forms of cancer drug resistance Drug screening in myeloma cell lines. (A) The overall screening results are displayed and expressed as survival index with results for 8226/Dox40 displayed on the Y axis and the parental RPMI 8226 cells on the X-axis. (B) Molecular structure and chemical properties of VLX40 (C) Activity of VLX40 against a cell line panel representing different forms of drug resistance. Cell survival was determined over 72 h using the FMCA assay in duplicate experiments. (D) Validation of VLX40 activity on 8226/Dox40 cells. Concentration-dependent effects of VLX40 on cell survival in RPMI 8226 (red line) and 8226/Dox40 (blue line) cell lines (triplicate samples). (E) Concentration-dependent effects of vincristine on cell survival in RPMI 8226 (red line) and 8226/Dox40 (blue line) cell lines. Survival in (D, E) was determined over 72 h using the FMCA assay. The results are expressed as percentage of the untreated control and presented as mean values +/- standard error of the mean (SEM) from three independent experiments.Back to article page