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Figure 4 | BMC Cancer

Figure 4

From: NK-cell-dependent killing of colon carcinoma cells is mediated by natural cytotoxicity receptors (NCRs) and stimulated by parvovirus infection of target cells

Figure 4

Effect of H-1PV infection on the phenotype of colon carcinoma and dendritic cells. (a) Colon carcinoma cells were buffer-treated (M) or H-1PV-infected (MOI=5 RU per cell), incubated for 24 h, and analyzed by flow cytometry for expression of MHC class I, MICB, and ULBP 2 molecules and NCR (NKp30, NKp44, and NKp46) ligands. Control mouse IgG and specific antibody staining profiles are shown by gray lines and black columns, respectively. The indicated values represent ∆MFI=MFI (positive)-MFI (isotype/negative control) for one representative experiment out of three. (b) Colo32 cells were mock-treated (M) or H-1PV-infected (MOI= 5RU/cell) and lysates prepared on day 1 p.i. Dendritic cells were then pulsed with lysate for 2days and thereafter, analyzed for expression of MHC class II molecules, and compared with untreated dendritic cells. Figure 4 (b) shows the means of data obtained from 3 donors. Control mouse IgG and specific antibody staining profiles are shown by grey lines and black columns, respectively. The indicated values represent ∆MFI = MFI (positive)-MFI (isotype/negative control).

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