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Table 3 Predictive value of each alternative technique compared with FISH expressed as HER2/CEN17 ratio in 3 categories in the overall population (n=766) and in the IHC 2+ subpopulation

From: SISH/CISH or qPCR as alternative techniques to FISH for determination of HER2 amplification status on breast tumors core needle biopsies: a multicenter experience based on 840 cases

Techniques

Population

Concordance (95%CI)

Sensitivity (95%CI)

Specificity (95%CI)

Positive predictive value (95%CI)

Negative predictive value (95%CI)

IHC

All (N=766)

96% [94-97]

91% [86-94]

98% [97-99]

95% [91-98]

96% [94-98]

SISH

All (N=498)

97% [96-99]

99% [95-100]

97% [94-98]

93% [88-96]

99% [98-100]

 

IHC 2+ (N=54)

89% [77-96]

80% [44-97]

91% [78-97]

67% [35-90]

95% [84-99]

CISH

All (N=108)

98% [93-100]

100% [89-100]

97% [91-100]

94% [80-99]

100% [95-100]

 

IHC 2+ (N=18)

100% [81-100]

100% [16-100]

100% [79-100]

100% [16-100]

100% [79-100]

qPCR

All (N=699)

95% [93-96]

89% [83-93]

97% [95-98]

92% [88-96]

95% [93-97]

 

IHC 2+ (N=86)

93% [85-97]

73% [45-92]

97% [90-100]

85% [55-98]

95% [87-98]

  1. CI: confidence interval.