Figure 7

Mifepristone-pretreated MDA-MB-231 (A), U87MG (B), LNCaP (C), and SKOV-3 (D) cells have delayed adhesion to extracellular matrix proteins. For all cell lines, treatment with cytostatic concentrations of mifepristone was administered for 72 h prior to plating. After treatment, cells were trypsinized and re-plated in a pre-coated plate at a density of 100,000 cells per well. FN: fibronectin, Col I: collagen I, Col IV: collagen IV, LN: Laminin, FG: Fibrinogen. Data shown in A-D represent one experiment that was repeated three times with a similar outcome. In E, SKOV-3 cells were cultured in the presence of vehicle (VEH) or mifepristone (MF) for 72 h. The cells were trypsinized and placed in plates that had been pre-coated with 0.1% fibronectin or bovine serum albumin (BSA) used as a negative control of adhesion. Adhesion was quantified as the number of cells attached per 20 X microscopic field. * p < 0.05 vs. VEH; ** p < 0.01 vs. VEH. Statistical analysis was done using two-way ANOVA followed by Bonferroni’s post-test. Bright field Images in F are representative of vehicle (VEH) or mifepristone (MF)-pretreated cells observed after 60 min of incubation (200 X).