Figure 6

Exposure to mifepristone impairs the ability of cancer cells to remain adherent. SKOV-3 (A), LNCaP (B), MDA-MB-231 (C) or U87MG (D) cells were plated at equal densities and treatment with vehicle (VEH) or mifepristone (MF)-containing media was administered for 72 h. Cells were then exposed to 0.025% trypsin/0.265 mM EDTA for 0.5, 2, or 4 min. The detached cells were removed, while those remaining adherent were fixed with 100% methanol and stained with 0.25% crystal violet (as shown in panel E for U87MG cells; 4 min trypsin exposure; 200 X). Stained cells were then imaged and counted via bright field microscopy. Adherent cells were quantified as a percent of control (0 min trypsin exposure). Data shown represent the mean ± s.e.m. of 3 independent experiments completed in triplicate. * p < 0.01 vs. VEH; ** p < 0.001 vs. VEH (A-D). Statistical analysis was done using two-way ANOVA followed by Bonferroni’s post-tests.