Figure 2

CXCR7 mediates the migration of LN229 and LN308 glioma cells towards SDF-1α in hypoxic conditions. shRNA-infected U87MG, LN229 and LN308 glioma cells were seeded in migration chambers in the presence or absence of 100 ng/ml (10 nM) of SDF-1α in the lower well. They were allowed to migrate for 8 h in normoxic or hypoxic conditions. Bar graphs indicate the average number of migrated cells per field. Error bars denote mean ± standard deviation. ^*P<0.001 versus normoxic control; ^**P<0.001 versus non-SDF-1α exposed cells; ^***P<0.001 versus SDF-1α exposed hypoxic cells. Bar graphs represent pooled data from two independent experiments. N, normoxia (20% O₂); H, hypoxia (1% O₂); white bars, shLacZ; grey bars, shCXCR7 S4; hatched bars, shCXCR7 S5.