Figure 4
From: Abnormal expression of Pygopus 2 correlates with a malignant phenotype in human lung cancer
Pygo2 siRNA reduces Pygo2 expression. Following transfection with Pygo2 siRNAs, Pygo2 expression was analyzed by Western blotting and RT-PCR. (A) Pygo2 protein bands were detected at approximately 50 KDa in untransfected and control SPC-A-1 cells. In contrast, cell lines transfected with sequence C showed reduced Pygo2 expression. (B) RT-PCR analysis indicated that Pygo2 mRNA was detected as a specific 170 bp band in untransfected and control SPC-A-1 cells. After transfection with Pygo2 siRNA, expression of Pygo2 mRNA was reduced. (C) Lanes 1–3 were siRNA-transfected cells transfected with empty plasmid. Lanes 4–6 were the siRNA-transfected cells transfected with Mus musculus Pygo2 cDNA plasmid. Protein bands representing Pygo2 at approximately 50 kDa were detected. Similar results were observed in A549 (D-F) and LTEP-a-2 (G-I) cells.