Figure 4

R2 increased and reactivated p53 transcriptional activity that is inhibited by FAK. A. Reactivation of p53 activity with p21 target by R2. The dual luciferase assay was performed in HCT116 p53−/− cells co-transfected with p53 and p21 promoter either without FAK plasmid without R2 or with 25 microM R2 treatment or with FAK plasmid without and with R2 treatment. The dual luciferase assay was performed as described in Materials and Methods. R2 compound reactivated p53 activity with p21 target inhibited by FAK. B. Reactivation of p53 activity with Mdm-2 target. The same assay as in Figure 4 A was performed with Mdm-2 promoter. R2 reactivated p53 activity with Mdm-2 target that was inhibited by FAK. C. Reactivation of p53 activity with Bax target. The same assay as in Figure 4A, B was performed with Bax promoter. R2 compound re-activated p53 activity with Bax target inhibited by FAK. *p<0.05, p53 activity with FAK versus no FAK, no R2 treatment, Student’s t-test.