Figure 1

DNR treatment reduces spleen weight in wt and p53-null mice. (A)  Cells isolated from the spleen of Trp53-wt and -null mice were treated for 7 h with DNR (10 μM) or IDA (0.5 μM). Cells were stained with AnnexinV and PI, and analysed by flow cytometry. Data are given as mean and SEM, n = 3. (B,C) Trp53-wt and -null mice were treated for 3 consecutive days with vehicle or 10 mg/kg DNR, and the spleen mass recorded and related to total animal weight at the given time points. The plots show relative spleen weight in treated animal to untreated animals. The arrowheads indicate days of DNR treatment. Diamonds represent untreated animals, and dots represent DNR-treated animals. The plots to the right show increase in spleen weight of DNR-treated animals 14 days after treatment. Note the difference in the scale between the left and right vertical axes. Each symbol represents one animal.