Figure 1

In situ hybridization and immunohistochemical analysis of GFRα1, GFRα3 and SDC3 mRNA and protein expression in benign breast disease and mammary carcinoma. A , In situ hybridization analysis. Up, low expression of GFRα1 and GFRα3 mRNA and high expression of SDC3 mRNA in mammary tissue derived from patients with benign breast disease; Bottom, high expression of GFRα1, GFRα3 and SDC3 mRNA in mammary carcinoma. B , Immunohistochemistry. Up, low expression of GFRα1 and GFRα3 protein and high expression of SDC3 protein in mammary tissue derived from patients with benign breast disease; Bottom, high expression of GFRα1, GFRα3 and SDC3 protein in mammary carcinoma. All images are counterstained with hematoxylin. Photomicrographs were captured at 200X magnification.