Figure 4

Anti-metastatic therapeutic effect of a systemic treatment with ssiRNAs and PEI. Nude mice were i.v. injected with B16-F10 cells, randomized and treated with glucose, negative control, survivin or cyclin B1 ssiRNAs and PEI (1 mg/kg, N/P=12.5). (a) Schematic representation of the treatment procedure. (b) Representative pictures of lungs excised from mice of the different treated groups. (c) Overall mean lung weight relative to the body weight of the mice. n = 8 animals per group. P values were calculated; * p<0.05; **p<0.01. (d) Representative images of Hematoxylin-Eosin stained lung sections after treatment with glucose, survivin or cyclin B1 ssiRNAs and PEI.