Figure 3

B16-F10 tumor xenograft growth inhibition upon systemic treatment of mice with ssiRNAs and PEI. (a) In vivo 5’-RACE analysis of RNA extracted from subcutaneous tumors 16 h after intra-tumoral injection of 0.6 mg/kg of negative control (2) and survivin or cyclin B1 (3) ssiRNA and PEI (N/P=8). As a control, 5’-RACE was also performed on RNA extracted from a tumor injected with glucose only (1). (b, c) Mice bearing tumor xenografts were treated intravenously every other day with glucose (diamond), negative control ssiRNA (triangle) and survivin (b) or cyclin B1 (c) ssiRNA (square) at 1 mg/kg, N/P=12.5. Tumor growth was monitored after each ssiRNA injection and represented as a mean tumor volume ± SEM. n = 10 animals per group. P values were calculated between control (glucose treated) group and specific ssiRNA groups; * p<0.05; **p<0.01.