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Figure 2 | BMC Cancer

Figure 2

From: Sticky siRNAs targeting survivin and cyclin B1 exert an antitumoral effect on melanoma subcutaneous xenografts and lung metastases

Figure 2

Effect of survivin and cyclin B1 inhibition on B16-F10 cell cycle progression . (a) Proliferation assay 48 h after transfection of ssiRNAs. The proliferation rate is represented as a percentage of cell growth relative to control untransfected cells. (b) Flow cytometer analysis of the cell cycle distribution of untransfected B16-F10 cells or 48 h after transfection with negative control, survivin or cyclin B1 ssiRNAs with PEI (50 nM, N/P=8). (c) Table presenting the percentage of cells in apoptosis (subG1 population) of 3 independent experiments determined after FACS analysis in the presence of propidium iodide 48 h after transfection with negative control and specific ssiRNAs with PEI (50 nM, N/P=8). (d) DAPI staining, 48 h after transfection with negative control, cyclin B1 or survivin ssiRNAs with PEI (50 nM, N/P=8) showing the presence of mega-nuclei and chromosomal aberrations in cells transfected with either cyclin B1 or survivin ssiRNAs.

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