The role of HGF/c-Met and EGFR family receptors in the HWF effect on HN-7 cells. (a) Cell lysates (5.6 μg protein per lane) from cells grown with 10% FBS or HWF were analyzed by western blotting with an anti-c-Met antibody. Relative expression of c-Met in HN-7 normalized to protein loading control is shown in the right panel. (b) Migration of HN-7 cells in the presence of 10% HWF and increasing amounts of a neutralizing anti-hHGF antibody (N=8). (c) The effect of HGF on cell scattering in the presence of 10% FBS, and inhibition of scattering stimulated by 10% HWF (N=8). Error bars represent SEM. (d) The effect of HWF on EGFR family activation. Cell lysates prepared from cells incubated with 10% FBS or HWF for 24 h were subjected to western blotting (10 μg protein per lane) with antibodies against p-EGFR and p-HER3. The band intensities were quantified with the AlphaView software package and normalized by Coomassie staining.