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Figure 5 | BMC Cancer

Figure 5

From: Sox2 suppresses the invasiveness of breast cancer cells via a mechanism that is dependent on Twist1 and the status of Sox2 transcription activity

Figure 5

The role of Sox2 and Twist1 in RR cells. Similar experiments were performed in MCF7-RU cells as described in Figure 4. (A) siRNA knockdown of Sox2 in MCF7-RR cells did not lead to a significant increase in invasiveness. Nevertheless, siRNA knockdown of Twist1 significantly decreased the invasiveness. Cells treated with double scramble siRNA were used as a negative control. Triplicate experiments were performed. A representative experiment is shown (mean ± standard deviation) (n = 3). One-way ANOVA was used to calculated statistics. (B) By western blot analysis, Sox2 siRNA and Twist1 siRNA treatment dramatically decrease the expression level of Sox2 and Twist1, respectively. (C) Cell viability was measured by the MTS assay. No significant change was observed between the negative control and various treatments.

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