Figure 1

Sox2 suppresses invasiveness in breast cancer cells. (A) MCF7 and ZR751 cells were treated with Sox2 siRNA before subjecting to invasion assay. siRNA knockdown of Sox2 significantly increased the invasiveness of MCF7 and ZR751cells. A scrambled siRNA sequence was used as a control and results were normalized to the control. Triplicate experiments were performed. A representative experiment is shown (mean ± standard deviation) (n = 3). Statistical significance was determined by Student's T-test. Western blots analysis showed that siRNA knockdown of Sox2 dramatically decreased the expression of Sox2 in MCF7 and ZR751. (B) Cell viability was measured by the MTS assay. siRNA knockdown did not significantly change the viability of MCF7 cells. Similar results were obtained from ZR751 cells. Triplicate experiments were performed (mean ± standard deviation) (n = 6). Statistical significance was determined by Student's T-test. (C) Enforced expression of Sox2 did not significantly change the invasiveness of MCF7. MCF7 cells transfected with empty vector were used as a control. Triplicate experiments were performed. A representative experiment is shown (mean ± standard deviation) (n = 3). (D) Cell viability was measured by the MTS assay. Enforced expression of Sox2 did not significantly change the viability of MCF7 cells. Triplicate experiments were performed (mean ± standard deviation) (n = 6).