Figure 1

Wnt-2 activation of Frizzled receptors. (A) TCF transcriptional activity was measured in 293T cells transfected with the indicated Frizzled expression vectors in the presence or absence of Wnt-2 cDNA and vector control. Cells were co-transfected with pTOPflash or pFOPflash and internal control plasmid pRL-TK. Experiments were performed in triplicate and the level of expression was shown as relative fold activation (TOP/FOP) (mean ± standard deviation). (B) Activation of Frizzled-8 by Wnt-2 was measured in 293T, 293 and NSCLC cell line A549. TCF activity was determined in the cells transfected with Frizzled-8, Wnt-2, both Frizzled-8 and Wnt-2, or vector control. Experiments were performed in triplicate.