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Table 1 Chromosome analysis of mouse bone marrow cells after exposure to RAN extract with or without lime

From: Induction of chromosome instability and stomach cancer by altering the expression pattern of mitotic checkpoint genes in mice exposed to areca-nut

Treatment pattern

Treatment days

Total spread scored

Chromosome no.

Aneuploidy

Premature sister

   

37

38

39

40

41

42

>60

% (Mean)

Chromatid separation %

Untreated

0

110

   

110

   

0

0

  

104

   

104

   

0

0

RAN

120

105

 

1

1

103

   

1.9

17.5

  

120

2

1

2

115

   

4.1

18.7

  

105

1

2

 

102

   

2.8 (2.9)

18.3 (18.2)

RAN + lime

 

100

2

2

1

95

   

5.0

31.7

  

105

2

2

3

98

   

6.6

28.6

  

110

3

2

1

103

 

1

 

6.4 (6.0)

28.3 (29.5)

           

p = 0.015a

RAN

180

101

1

2

1

97

   

3.9

18.0

  

124

2

2

1

119

   

4.0

17.7

  

114

3

2

1

108

   

5.3 (4.4)

19.4 (18.4)

RAN + lime

 

112

3

3

3

103

   

8.0

32.6

  

105

2

2

1

98

 

1

1

6.6

34.9

  

110

4

2

 

101

 

2

1

8.2 (7.6)

35.6 (34.4)

           

p = 0.002a

RAN + lime

220

232

6

9

12

194

7

2

2

16.4

8.2

(with advanced tumor)

256

234

5

4

7

208

5

2

3

11.1 (13.8)

7.1 (7.6)

  1. a statistically significant in paired t-test; two-tailed p value was shown.