Figure 5
Upper panel- Representative western blotting detection of p65, p53, securin, bax and β-actin in mouse bone marrow (BM), esophagus and stomach cells after exposure with RAN extract with or without lime. For BM, cells were collected after 60, 120 and 180 days, whereas for esophagus and stomach, cells were collected only after 180 days of exposure. β-actin was used as loading control. Lower panel- Quantitative densitometric analysis of the level of proteins of the above mentioned genes in bone marrow, esophagus and stomach cells after 180 days of exposure was shown. The values are the mean ± SD of three independent experiments. The values are normalized to respective β-actin values. * p < 0.05; ** p < 0.01 Significantly different compared with negative/positive control (as determined by paired t-test).