Figure 4
Effects of intratumoural Ad-NDRG2 injections on the growth of Eca-109 cells xenografted into mice. Eca-109 cells (5 × 106) were injected into the upper hind limb of athymic nude mice and allowed to grow until the tumour volume reached 200 mm3. Mice then received intratumoural Ad-NDRG2 or Ad-LacZ injections (at 50 MOI) or PBS (20 μl as control) every 3 days. A. Tumour growth curve. The tumour growth was assessed every 3 days until Day 24 of treatment by measuring two perpendicular diameters and calculating the volume in mm3. Statistical analysis was performed on Day 24 values only, using a one-way ANOVA and Student’s t-test. ** indicates P < 0.01 as compared to the control. B. Representative photographs of xenografted tumours are shown. C. Intratumoural the protein expression was assessed by cyclinD1-immunolabeling (400 × magnification) or Ki67-immunolabeling (400 × magnification) on paraffin-embedded Eca-109 cell tumour sections. Representative images are shown. Statistical analysis was carried out using a one-way ANOVA. The results are shown as the mean ± SD of three different fields per mouse from a total of six mice. **P < 0.01.