Skip to main content
Figure 2 | BMC Cancer

Figure 2

From: Low expression of N-myc downstream-regulated gene 2 in oesophageal squamous cell carcinoma correlates with a poor prognosis

Figure 2

Up-regulation of NDRG2 expression by Ad-NDRG2 in Eca-109 cells. A. HEEC cells (a normal human oesophageal epithelial cell line) and five ESCC cell lines (EC8712, KYSE30, Eca-109, KYSE70 and KYSE150) were collected for protein extraction and analysed for NDRG2 expression using western blot. B. Subcellular localisation of NDRG2 in Eca-109 cells. Eca-109 cells were fixed and incubated with a primary anti-NDRG2 monoclonal antibody and a CY3 secondary antibody. Red fluorescence (NDRG2) was observed in the cytomembrane and cytoplasm of Eca-109 cells. Blue fluorescence indicates DAPI nuclear staining. C. Immunofluorescence showed the efficiency of Ad-NDRG2 infection (red). D. Western blot analysis of NDRG2 in Eca-109 cells infected with Ad-NDRG2. Equal amounts of protein (80 μg/lane) were subjected to western blot analysis as described in the Methods.

Back to article page