Figure 6

RPS4X depletion increases cipslatin resistance in OVCAR-3 and SK-OV-3 cells. A, One example of a western blot showing expression of RPS4X in untransfected OVCAR-3 and SK-OV-3 cells. β-actin is used as a loading control. B, Histogram presenting the ratio of RPS4X signal over β-actin signal from western blots. Experiments were performed in duplicate. C, Cisplatin dose response curves for transfected OVCAR-3 cells. D, Cisplatin dose response curves for transfected SK-OV-3 cells. Cells were transfected with the indicated siRNA molecules. Cisplatin dose response curves were determined by the sulforhodamine B colorimetric assay. E, Histogram representing the IC50 of OVCAR-3 transfected cells (from at least three transfections for each siRNA sequences) calculated from the drug response curves in A. Error bars represent the standard deviation. (Unpaired student t-test: P = 0.0084 for siRPS4X-A vs siCTRL and P = 0.025 for siRPS4X-D vs siCTRL). F, Histogram representing the IC50 of SK-OV-3 transfected cells (from at least three transfections for each siRNA sequences) calculated from the drug response curves in B. Error bars represent the standard deviation. (Unpaired student t-test: P = 0.00066 for siRPS4X-A vs siCTRL and P = 0.0001 for siRPS4X-D vs siCTRL). IC50 were estimated as described in materials and methods.