Figure 5

Apoptotic and necrotic events in RPS4X-depleted cells were assessed in the presence of cisplatin by flow cytometry with Annexin V/PI staining. Cells were transfected with the indicated siRNA sequences (siControl and siRPS4X-A) and 48 hours later cells were treated for 48 hours with the indicated concentration of cisplatin. In each graph, the P1 section (top left) represents necrotic cells, the P2 section (top right) represents both apoptotic and necrotic cells, the P3 section (bottom left) represents healthy cells, and the P4 section (bottom right) represents apoptotic cells. The number and percentage of cells in each section are indicated below each graph.