Figure 3

Western blot analysis of total protein extracts from OVCAR-3 and SK-OV-3 cell lines transfected with different siRNA sequences. A, OVCAR-3 cells were transfected with the indicated siRNA molecule and 48 hours later total protein extracts were purified, loaded on SDS/PAGE gel, and membranes were hybridized with anti-YB-1, anti-RPS4X, and anti-β-actin. Representative blots are presented. B, Histograms presenting the ratio of YB-1 or RPS4X signal over β-actin signal from the western blots in panel A. C, SK-OV-3 cells were transfected with the indicated siRNA molecule and 48 hours later total protein extracts were purified, loaded on SDS/PAGE gel, and membranes were hybridized with anti-YB-1, anti-RPS4X, and anti-β-actin. Representative blots are presented. D, Histograms presenting the ratio of YB-1 or RPS4X signal over β-actin signal from the western blots in panel C. All experiments were performed in duplicate.