Figure 5

Effect of AG1478 or/and AG1024 plus irradiation on total and phosphor-Akt and -Erk1/2 in MDA-MB-468 cells. MDA-MB-468 cells were exposed to AG1478 (10 μM for 1 hour) and/or AG1024 (10 μM for 1 hour), and were irradiated at 4Gy for 24 hours. Total and phosphorylated Akt, total and phosphorylated Erk1/2 were examined. The density of each band were shown under the p-Akt and p- Erk1/2 panel, these represent phosphor-protein relative to total protein, the phosphor-protein/total protein values were then normalized to the value for control cells, the cells were assigned the value = 1. RT = radiotherapy.