Figure 5

The metastasis of MC38/EGFP colon cancer cells to lymph nodes in mice treated with 5-FU and PRI-2191 or PRI-2205. Mice bearing s.c. MC38/EGFP colon cancer cells were i.p. treated with 5-FU at a dose of 75 mg/kg/day from days 19 to 29 alone and in combination with vitamin D analog PRI-2191 at a dose of 1 μg/kg/day or PRI-2205 at a dose of 10 μg/kg/day. Both analogs were administrated s.c., three times a week from days 19 to 56. The mean fluorescence intensity value of the green fluorescent protein which is directly proportional to the number of MC38/EGFP cells in lymph nodes isolated from mice on the 59th day of the experiment was calculated. The number of mice in each group – 7. Statistical analysis was performed using the Kruskal-Wallis ANOVA Multiple Comparisons P values (2-tailed) test. ^*** P < 0.001 – 5-FU + PRI-2191 compared to 5-FU; ^** P < 0.01 – 5-FU + PRI-2191 compared to 5-FU + PRI-2205.