mRNA mini-array to uncover ID3-related genes. (A) Expression of 66 genes related to cellular invasion and metastasis after ID3 knockdown using siRNA in D283 cells. (B) RT-PCR of ID3 and the 7 genes with considerable initial expression levels and expression level changes of greater than 2-fold. (C) Representative images of immunohistochemistry of ID3 and 7 proteins in seeding-negative and seeding- positive medulloblastomas (200×). Note that TNC is mainly expressed in the tumor stroma (arrow) than tumor cell clusters where ID3 immunoreactivity is observed.