Figure 3
From: ID3 contributes to cerebrospinal fluid seeding and poor prognosis in medulloblastoma
In vito cellular effects of ID3 knockdown by siRNA in D283 cells. (A) Cellular viability was assessed using CCK assays. There was a significant reduction of cell viability (B) Cell proliferation was significantly decreased, assessed using BrdU assays. (C) Apoptosis was assessed using TUNEL staining (40×). (D) Quantification of TUNEL-positive cells revealed a significant increase of apoptosis after siRNA treatment. (E) There was no difference in senescence, assessed using β-gal staining. (F and G) Cell cycle analysis using FACS showed that G2 phase and sub-G1 fractions were increased after siRNA treatment. Migratory ability was assessed using transwell migration assays. (H) Representative field images (100×) and (I) the quantification of migrated cells revealed suppression of cell migration by ID3-siRNA. Error bars represent standard deviations.