Figure 4

Transformation of gastric cancer cells is associated with altered SIRT1 and miR-204 expression. (A) An altered morphology is associated with transformation. Representative images display morphological changes from HGC to HGC-T (treated with 10 ng/ml TGF-β1for 21 days). Scale bar ,100μm. Vimentin (B) and E-cadherin (C) mRNA levels in transformed cells transfected with 50 nM miR-204 precusor for 72h. (D) Western bloting of E-cadherin and Vimentin protein levels. E-cadherin and Vimentin levels in HGC-T transfected with 50 nM miR-204 precusor or SIRT1 SiRNA for 48h. Vimentin (E) and E-cadherin (F) mRNA levels in transformed cells transfected with 50 nM SIRT1 SiRNA for 72h. Representative results from 3 independent experiments. Real-time RT-PCR results for mRNA levels were normalized to GAPDH mRNA. **P < 0.01. *P < 0.05.