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Table 2 Source, clone and dilution of Primary antibodies

From: Profiling of normal and malignant breast tissue show CD44high/CD24lowphenotype as a predominant stem/progenitor marker when used in combination with Ep-CAM/CD49f markers

  Antibody Company clone Added volume (per 1-6 x 10^6 cells) or dilution
1 CD45-AC BD 2D1 5 μL
2 MUC-1-FITC BD HMPV 5 μL
3 CD31-FITC BD WM59 5 μL
4 CD49f-PE, FITC, PE-Cy5 BD GoH3 7 μL
5 EPCR (CD201) PE BD RCE-252 5 μL
6 SSEA-4-Percp-Cy5.5 Ebioscience 5E10 20 μL
7 ABCG2-Percp-Cy5.5 or APC Biolegend 5D3 3 μL
8 CD24-PE-Alex 610 Invitrogen SN3 5 μL
9 CD24 FITC, or PE BD ML5 5 μL
10 CD10-PE-Cy7 BD HI10a 5 μL
11 CD117 (c-Kit)-PE-Cy7 Biolegend 104D2 5 μ
12 CXCR-4 (CD184) PE-Cy7 Biolegend 12G5 5 μ
13 Ep-CAM-APC, FITC, or PE Miltenyi Biotec HEA-125 7 μL
14 CD44-APC-Cy7 Biolegend IM7 1 μL
15 CD31-Alex 760 Invitrogen WM59 1 μL
16 Estrogen alpha-unlabelled Abcam polyclonal 1/300 dilution
  1. * Indirectly labeled with secondary anti-rabbit pacific blue antibody.