Table 2 Source, clone and dilution of Primary antibodies
| Â | Antibody | Company | clone | Added volume (per 1-6 x 10^6 cells) or dilution |
|---|---|---|---|---|
1 | CD45-AC | BD | 2D1 | 5 μL |
2 | MUC-1-FITC | BD | HMPV | 5 μL |
3 | CD31-FITC | BD | WM59 | 5 μL |
4 | CD49f-PE, FITC, PE-Cy5 | BD | GoH3 | 7 μL |
5 | EPCR (CD201) PE | BD | RCE-252 | 5 μL |
6 | SSEA-4-Percp-Cy5.5 | Ebioscience | 5E10 | 20 μL |
7 | ABCG2-Percp-Cy5.5 or APC | Biolegend | 5D3 | 3 μL |
8 | CD24-PE-Alex 610 | Invitrogen | SN3 | 5 μL |
9 | CD24 FITC, or PE | BD | ML5 | 5 μL |
10 | CD10-PE-Cy7 | BD | HI10a | 5 μL |
11 | CD117 (c-Kit)-PE-Cy7 | Biolegend | 104D2 | 5 μ |
12 | CXCR-4 (CD184) PE-Cy7 | Biolegend | 12G5 | 5 μ |
13 | Ep-CAM-APC, FITC, or PE | Miltenyi Biotec | HEA-125 | 7 μL |
14 | CD44-APC-Cy7 | Biolegend | IM7 | 1 μL |
15 | CD31-Alex 760 | Invitrogen | WM59 | 1 μL |
16 | Estrogen alpha-unlabelled | Abcam | polyclonal | 1/300 dilution |