Effects of SiWee1 on cell cycle distribution and on proteins involved in cell cycle regulation. SW-954 and CAL-39 cells were transfected with either SiCtr or SiWee1 (25 nM) and harvested after 48 hrs. A. Cells were stained with Hoechst and cellular DNA content was measured by Flow cytometry. B. Western blot analysis was conducted with the indicated antibodies. α-tubulin was used as loading control. Data are representative of three independent biological experiments.