DNA damages and reduced viability following SiWee1 transfection in vulva cells. SW-954 and CAL-39 cells were transfected with either SiCtr or SiWee1 (25 nM) and harvested/measured after 48 hrs. A. Western blot analysis was conducted with the indicated antibodies. α-tubulin was used as loading control. B. Effect of SiWee1 on cell viability as measured by MTS assay. Error bars represent the standard deviation from four independent experiments.