Figure 4

Synergistic growth inhibition using SAHA with fenretinide and with conventional chemotherapy in rhabdoid tumor cell lines. A, B, C. HDACi (SAHA, M344, TSA) were used in concentrations ranging from 0.01 μM to 100 μM. In single compound experiments fenretinide was used in the same increasing concentration (0.01 μM to 100 μM). In the combined approach we used HDACi (SAHA, M344, TSA) from 0.01 μM to 100 μM in combination with 10% fenretinide (0.001 μM to 10 μ). Median effect plots show that, SAHA and other HDACi (M344 and TSA) act strongly synergistic with the cyclinD inhibitor fenretinide (for CI-values see also table 1). D, E, F. Three different rhabdoid tumor cells lines (A204, G401, BT16) were treated with SAHA, doxorubicin or combinations of both compounds for 72 h and were analysed using MTT-assays. Median effect blots demonstrate that conventional chemotherapy (doxorubicin) acts synergistically with SAHA on inhibiting cell proliferation.