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Figure 5 | BMC Cancer

Figure 5

From: Mitochondria-targeted vitamin E analogs inhibit breast cancer cell energy metabolism and promote cell death

Figure 5

Intracellular accumulation of Mito-ChM in MCF-7, MDA-MB-231 and MCF-10A cells. (A) HPLC-EC chromatograms (dominant channels) of the mixture of standards (100 μM) of α-tocopherol and Mito-ChM, and of extracts from cells treated for 4 h with 10 μM Mito-ChM (left panel). Quantitative data on intracellular concentration of Mito-ChM after normalization to protein content (right panel). (B) Same as in panel A, but after a 4 h treatment with 10 μM Mito-ChM, medium was changed and cells incubated further for another 24 h in culture medium in the absence of Mito-ChM. Chromatogram of standards represents a mixture of α-tocopherol and Mito-ChM (10 μM each). (C) Same as in panel A, but cells were treated for 48 h with 1 μM Mito-ChM. Chromatogram of standards represents a mixture of α-tocopherol and Mito-ChM (1 μM each). (D) Same as in panel A, but cells were treated for 4 h with 10 μM Mito-ChMAc. (E) HPLC-MS/MS chromatograms [MRM transitions: 679.1 → 515.0 for Mito-ChM (upper traces) and 721.1 → 415.0 for Mito-ChMAc (lower traces)] of the mixture of standards (100 μM) of Mito-ChM and Mito-ChMAc, and of extracts from cells treated for 4 h with 10 μM Mito-ChMAc (left panel). Quantitative data on intracellular concentrations of Mito-ChM and MitoChMAc after normalization to protein content (right panel). (F) Intracellular levels of Mito-ChM in MDA-MB-231 cells incubated for 2 and 4 h with 10 μM Mito-ChM or Mito-ChMAc (left panel). Right panel shows similar data, but after 4 h incubation with the compounds, cells were incubated for 24 h in culture medium alone.

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