Figure 3

Effects of Mito-ChM on basal OCR and bioenergetics functions in MCF-7 and MCF-10A cells. (A) Experimental protocol for bioenergetic functional assay. To determine the mitochondrial and glycolytic function of MCF-7 and MCF-10A cells in response to Mito-ChM (1–10 μM), we used the bioenergetic functional assay previously described (4,25). After seeding and treatment, MCF-7 cells and MCF-10A cells were subsequently washed with complete media (MEM-α for MCF-7 and DMEM/F12 for MCF-10A) and either assayed immediately, or returned to a 37°C incubator for 24, 48, or 72 h. The relative time of treatment and post-treatment incubation that corresponds to the appropriate figures is indicated. (B) MCF-7 and MCF-10A cells were assayed for OCR immediately after treatment with Mito-ChM (1–10 μM) for 4 h, (C) after incubation without Mito-ChM for an additional 24 h, (D) after additional incubation without Mito-ChM for 48 h, and (E) after additional incubation without Mito-ChM for 72 h.