Figure 1

The cytotoxic effect of Mito-ChM in breast cancer and non-cancerous cells. Nine different breast cancer cells and MCF-10A cells were treated with Mito-ChM at the indicated concentrations (0.5-20 μM) for 24 h, and cell death was monitored in real time by Sytox Green staining. Data shown are the means ± SEM for n = 4. Real time cell death curves were plotted in panel A for MCF-7 (left), MDA-MB-231 (middle) and MCF-10A cells (right). Panel B shows the titration of breast cancer and non-cancerous cells with Mito-ChM, and the extent of cell death observed after 4 h treatment is plotted against Mito-ChM concentration. Solid lines represent the fitting curves used for determination of the EC₅₀ values, indicated in each panel.