Figure 2

Anti-invasive and anti-migratory potential of TAM and CXB in MCF7 and MDA-MB-231 cells. (A) Representative hematoxylin- and eosin-stained cell images migrating into the wounded area in an in vitro wound healing assay at times 0 h and 48 h. Scale bars, 100 μm. (B) Quantification of wound-healing results. Data are means ± SE of three random widths along the wound. P < 0.05. (C) Representative photomicrographs of Boyden chamber assays of MDA-MB-231 cell invasion through Matrigel. Cells were stained with hematoxylin- and eosin. (D) Top: Gelatinolytic activity of MMP-2 in MCF-7 cells and MDA-MB-231 cells treated for 48 h. Bottom: Densitometric analysis of MMP-2 protein levels in gelatin blot. Data are means ± SE of three independent experiments. P < 0.05 (t-test).T + C, TAM plus CXB; UT, untreated cells.