Immunohistochemical detection of cancer - testis antigens and NY-BR-1 in primary breast cancer tissue microarrays. A: Example of moderate staining of MAGE A1 in approximately 80% of the tumor cells. The staining is restricted to the cytoplasm. B: Strong nuclear and cytoplasmic expression of MAGE A1. C: Extensive strong nuclear and cytoplasmic M3H67 immunoreactivity. D: Strong, mainly cytoplasmic and occasionally nuclear 57B immunoreactivity. E: Extensive strong nuclear and cytoplasmic detection of MAGE A (6C1). F: Focal strong, mainly cytoplasmic staining of approximately 20% of tumor cells for GAGE. G: Strong extensive cytoplasmic and occasionally nuclear staining of NY-ESO-1. H: Strong cytoplasmic NY-BR-1 staining of approximately 80% of cells with scarce nuclear detection (40x objective).