Figure 1

EphB4 overoverexpression in MPM. (A) A panel of MPM tissues was stained with EphB4-specific antibody MAb131, and EphB4 expression level was scored and summarized. Moderate and strong expression was considered overexpression. (B) Representative images demonstrating EphB4 expression patterns in epithelioid and sarcomatoid subtypes. (C) 293T cells grown on 8-well chamber slide were transfected with human EphB4 overexpresion vector or empty vector pCDNA3.1. 2 days after transfection, cells were fixed with 4% paraformaldehyde and stained with MAb131 that was also used for staining in (A). Nuclei were counter-stained with DAPI.