Figure 8
The effect of EGF stimulation on phosphorylated protein expression. PLC/PRF/6 cells were stimulated with 10 ng/ml EGF for the indicated times, lysed and analyzed by western blotting (A). Sk-Hep1 cells were stimulated with 200 ng/ml EGF for the indicated times, lysed and analyzed by western bloting with the indicated antibodies (C). The expression ratio of phosphorylated protein to β-actin was quantified by ImageJ software respectively. Results represented the mean (±SD) of three experiments. * p < 0.05 as compared with the control (student’s t-test) (B and D).