Figure 2

Methylation specific PCR. Legend: A) Example of a methylation specific PCR: KiSS-1. Samples are noted above the image and primer specificity is declared as U, specific for unmethylated template, or M, specific for methylated template. 3 distinct signal patterns were observed: either only the U- or the M-band was present, or both bands were present. Cell lines were thus identified as unmethylated (e.g. MPanc96), methylated (e.g. PaTu8988S) or heterogeneously methylated (e.g. CAPAN2). B) MSP-analysis for all cell lines and genes. Each column represents a gene and its methylation status in a cell line is indicated by partially filled circles. Methylation status for each cell line was determined as illustrated in Figure 3A for the example for KiSS-1. *different results for MSP- vs. BSP-analysis (compare to Figure 3).