Figure 9

Loss of p63 expression in basal cells detected by fluorescence-based immunostaining. A set of three adjacent human prostate tissue sections were double immunostained for CK34βE12 and p63, the antigen-antibody complexes were distinguished with secondary antibodies labeled with different fluorophores (A-F) or a red chromogen (G-H). Note that the basal cells near the up-middle have substantially reduced expression of both CK34βE12 and p63. A and C: 150X. B and D. A higher (400X) of A and C, respectively.