Figure 2

Antioxidants Vit C and BHA prevent estrogen-mediated inhibition of OGG1. (A) Real-time PCR data are presented as a bar graph to show expression levels of OGG1 mRNA in mammary tumors and mammary tissues of rats treated with E2, Vit C or BHA in presence or absence of E2 for 240 days as described in “Methods” section. OGG1 mRNA expression data were normalized to cyclophilin as an internal control. These data are reported as an average of values obtained from at least 5 different animals ± SEM, compared to age-matched control mammary tissues. (B) Female ACI rats were treated with E2, Vit C or BHA in presence or absence of E2 for 240 days as described in “Methods” section. At the end of the experiment, mammary tissues and mammary tumors were collected, homogenized and used for OGG1 western blot analysis. The bar graph represents OGG1 protein fold change (mean ± SEM) in mammary tumors and mammary tissues from at least 5 different animals from each treatment group compared to age-matched control mammary tissues. ‘*’ indicates p value <0.05 compared to age-matched control mammary tissues.