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Table 3 Comparison between FISH and FACS data to evaluate HAC induced by drug treatment

From: A new assay for measuring chromosome instability (CIN) and identification of drugs that elevate CIN in cancer cells

Drug FISH FACS Rate of HAC loss
  % Cells with HAC % Cells with EGFP per cell division**
No drug 86 (100)* 85.1 ± 0.9 13 × 10-3
Taxol 59 (139) 59.5 ± 0.9 597 × 10-3
Ixabepilone 71 (116) 68.0 ± 0.1 396 × 10-3
Docetaxel 74 (101) 79.1 ± 1.8 133 × 10-3
Peloruside A 68 (71) 67.4 ± 0.9 411 × 10-3
Nocodazole 79 (77) 80.3 ± 0.9 107 × 10-3
SAHA 79 (160) 84.3 ± 1.2 12 × 10-3
VP16 (Etoposide) 86 (156) 80.7 ± 0.9 96 × 10-3
Reversine*** 77 (71) 79.0 ± 0.5 176 × 10-3
ZM-447439*** 75 (75) 70.4 ± 0.5 374 × 10-3
  1. * In parentheses, the number of metaphases screened for the presence or absence of the HAC.
  2. ** Rates of the HAC loss after drug treatment were calculated from FACS data using the formula P Treated = P 0 2 R Drug 2 n 2 2 R Normal 2 n 3 .
  3. *** A real rate of HAC loss may be a little bit lower after treatment by inhibitors of Aurora B and MPS1 because reversine and ZM-447439 do not completely block cell divisions.