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Table 3 Comparison between FISH and FACS data to evaluate HAC induced by drug treatment

From: A new assay for measuring chromosome instability (CIN) and identification of drugs that elevate CIN in cancer cells

Drug

FISH

FACS

Rate of HAC loss

 

% Cells with HAC

% Cells with EGFP

per cell division**

No drug

86 (100)*

85.1 ± 0.9

13 × 10-3

Taxol

59 (139)

59.5 ± 0.9

597 × 10-3

Ixabepilone

71 (116)

68.0 ± 0.1

396 × 10-3

Docetaxel

74 (101)

79.1 ± 1.8

133 × 10-3

Peloruside A

68 (71)

67.4 ± 0.9

411 × 10-3

Nocodazole

79 (77)

80.3 ± 0.9

107 × 10-3

SAHA

79 (160)

84.3 ± 1.2

12 × 10-3

VP16 (Etoposide)

86 (156)

80.7 ± 0.9

96 × 10-3

Reversine***

77 (71)

79.0 ± 0.5

176 × 10-3

ZM-447439***

75 (75)

70.4 ± 0.5

374 × 10-3

  1. * In parentheses, the number of metaphases screened for the presence or absence of the HAC.
  2. ** Rates of the HAC loss after drug treatment were calculated from FACS data using the formula P Treated = P 0 2 − R Drug 2 n 2 2 − R Normal 2 n 3 .
  3. *** A real rate of HAC loss may be a little bit lower after treatment by inhibitors of Aurora B and MPS1 because reversine and ZM-447439 do not completely block cell divisions.