|
Drug
|
FISH
|
FACS
|
Rate of HAC loss
|
|---|
| |
% Cells with HAC
|
% Cells with EGFP
|
per cell division**
|
|---|
|
No drug
|
86 (100)*
|
85.1 ± 0.9
|
13 × 10-3
|
|
Taxol
|
59 (139)
|
59.5 ± 0.9
|
597 × 10-3
|
|
Ixabepilone
|
71 (116)
|
68.0 ± 0.1
|
396 × 10-3
|
|
Docetaxel
|
74 (101)
|
79.1 ± 1.8
|
133 × 10-3
|
|
Peloruside A
|
68 (71)
|
67.4 ± 0.9
|
411 × 10-3
|
|
Nocodazole
|
79 (77)
|
80.3 ± 0.9
|
107 × 10-3
|
|
SAHA
|
79 (160)
|
84.3 ± 1.2
|
12 × 10-3
|
|
VP16 (Etoposide)
|
86 (156)
|
80.7 ± 0.9
|
96 × 10-3
|
|
Reversine***
|
77 (71)
|
79.0 ± 0.5
|
176 × 10-3
|
|
ZM-447439***
|
75 (75)
|
70.4 ± 0.5
|
374 × 10-3
|
- * In parentheses, the number of metaphases screened for the presence or absence of the HAC.
- ** Rates of the HAC loss after drug treatment were calculated from FACS data using the formula .
- *** A real rate of HAC loss may be a little bit lower after treatment by inhibitors of Aurora B and MPS1 because reversine and ZM-447439 do not completely block cell divisions.
