Table 1 Real-time PCR primer sequences
Gene | Primer | Sequence of primer | Length of sequence (Base) | Length of amplified product (bp) |
|---|---|---|---|---|
G6PD | G6PD-F | 5′TGAGCCAGATAGGCTGGAA3′ | 19 | 225 |
|  | G6PD-R | 5′TAACGCAGGCGATGTTGTC3′ | 19 |  |
CyclinD1 | CyclinD1-F | 5′CGGTAGTAGGACAGGAAGTT3′ | 20 | 120 |
|  | CyclinD1-R | 5′CTGTGCCACAGATGTGAAGT3′ | 20 |  |
Fas | Fas-F | 5′GCCACCGACTTTAAGTTTGC3′ | 20 | 102 |
|  | Fas-R | 5′CGAGCTCACTTCCTCATCCT3′ | 20 |  |
STAT3 | STAT3-F | 5′CACCCGCAATGATTACAGTG3′ | 20 | 126 |
|  | STAT3-R | 5′CGGTCTGACCTCTTAATTCG3′ | 20 |  |
STAT5 | STAT5-F | 5′CACCCGCAATGATTACAGTG3′ | 20 | 126 |
|  | STAT5-R | 5′CGGTCTGACCTCTTAATTCG3′ | 20 |  |
β-actin | β-actin-F | 5′TGGCACCCAGCACAATGAA3′ | 20 | 186 |
|  | β-actin-R | 5′CTAAGTCATAGTCCGCCTAGAAGCA3′ | 25 |  |