Notch1 is up-regulated in ICC tissues and ICC cell lines. (A) The expression of ICN (the intracellular domain of Notch1) is elevated in primary ICC tumors (T) compared with ICC tumor-adjacent tissues (N) examined by Western blotting. (B) The expression of Notch1 mRNA in each of the primary ICC tumor (T) and ICC noncancerous tissue (N) pairs from the same patient by reverse transcription-PCR. β-actin was used as a loading control. (C) The expression of ICN protein is elevated in ICC cell lines.