Figure 3

Effect of the pan-caspase inhibitor Z-VAD-FMK on equol and 4-OHT induced MCF-7 cell apoptosis. Cells were plated in 60-mm plates and treated with equol (100 μM) and 4-OHT (10 μM) for 72 h. Cell apoptosis was evaluated using annexin-V Alexa Fluor® 488/PI staining and the Tali™ Image-based Cytometer. Each experimental group was repeated in triplicates and data are representative of three individual experiments. The bars correspond to the SEM. * P _{Equol vs [Z-VAD-FMK+Equol]}  = 0.014; ** P _{4-OHT vs [ZVAD-FMK+4-OHT]} =0.012; *** P _{[Equol+4OHT] vs [Z-VAD-FMK+Equol+4-OHT]} =0.017.