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Figure 1 | BMC Cancer

Figure 1

From: Equol enhances tamoxifen’s anti-tumor activity by induction of caspase-mediated apoptosis in MCF-7 breast cancer cells

Figure 1

Comparison of the effect of equol and 4-OHT on MCF-7 cell viability. (A) Cells (3 × 103/well) were plated in 96-well plates and treated with different concentrations of equol and 4-OHT, individually or combined. After 24, 48 and 72 h, cell viability was evaluated using the MTT assay. The OD reading at 570 nm was proportional to cell viability. * P < 0.05, ** P < 0.005 and *** P <0.0005. P Equol (100 μM) vs control = 0.003; P 4-OHT(10 μM) vs control = 0.002; P[ Equol (100 μM)+4-OHT (10 μM)] vs control = 0.0003. Dose response curves for equol (B) or 4-OHT (C) effect on MCF-7 cell viability. Cells (3 × 103/well) were seeded in 96-well plates and treated with different concentrations of equol (B) or 4-OHT (C). After 72 h, cell viability was evaluated using the MTT assay. The data are expressed as percentage change in viability in comparison to the vehicle treated control group. Each experimental group was repeated in quadruplicates and data are representative of three individual experiments. Bars correspond to the standard error of mean (SEM).

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