IGF-I and hyperglycaemia enhance pAMPK
phosphorylation. (A-C) BxPC-3 and (D-F) MIAPaCa-2 cells were treated in normal (5 mM) or high (25 mM) glucose-containing SFM, with or without 10 mM metformin for 24 h. Cells were spiked with IGF-I (100 ng/ml) as indicated for the final 15 min prior to being lysed and processed for pAMPKThr172, pAMPKSer485 and AMPK abundance by Western immunoblotting. GAPDH is shown as loading control. One representative blot is shown. Graphs display densitometry measurements of relative levels at 5 mM (black bars) or 25 mM (striped bars) glucose conditions and represent means ± SE of two (B) or three (A, C-D) independent experiments.